解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80187-3
更新日期:1985-01-01 00:00:00
abstract::The effect of oligosaccharide carrying lipids on membrane fluidity has been investigated. Gangliosides GM1 and GQ1 were reconstituted into phosphatidylcholine bilayer membranes at low concentrations (less than 5 mol%). A strong fluidizing effect was observed leading to a suppression of the phase transition temperature...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80214-3
更新日期:1985-01-01 00:00:00
abstract::The isolation and primary structure of intestinal neuropeptide Y (NPY) is described. The peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for NPY. The amino acid sequence of this peptide is: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala- Arg-Ty...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80216-7
更新日期:1985-01-01 00:00:00
abstract::The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochond...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80622-5
更新日期:1984-12-10 00:00:00
abstract::Resonance Raman (RR) spectra of the monomeric cyanomethaemoglobin CTT III from insect larvae of Chironomus thummi thummi are shown for the range of 200-550 cm-1. By iron and cyanide isotope exchange a line varying between 307 cm-1 for 57Fe-13C15N and 311 cm-1 for 54Fe-12C14N, has been assigned to the Fe-N epsilon stre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81233-8
更新日期:1984-12-03 00:00:00
abstract::The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81302-2
更新日期:1984-11-19 00:00:00
abstract::The pure lipoxygenase from reticulocytes converts 5,15-di-HETE at 2 degrees C to product(s) showing a characteristic UV spectrum with maxima of strong absorbance at 300 and 316 nm and shoulders at 285 and 350 nm. Their formation was completely prevented by the lipoxygenase inhibitors, ETYA and NDGA, by heating of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81294-6
更新日期:1984-11-19 00:00:00
abstract::In several reactions catalyzed by highly purified peptide elongation factor 1 from rabbit reticulocytes, GTP may be fully replaced by CTP but not by ATP or UTP. This holds true for the factor-dependent binding of aminoacyl-tRNA to ribosomes, GTPase activity, GTP-dependent autophosphorylation of the factor protein and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80992-8
更新日期:1984-11-05 00:00:00
abstract::The dynamics of changes in membrane potential, cytosolic free Ca2+, [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80972-2
更新日期:1984-11-05 00:00:00
abstract::We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80975-8
更新日期:1984-11-05 00:00:00
abstract::The maximal activities of the key glycolytic enzymes hexokinase and 6-phosphofructokinase, were reduced in brown adipose tissue in db/db mice compared to their lean littermates. Treatment of db/db mice with the thermogenic beta-adrenoceptor agonist, BRL 26830, restored normoglycaemia. The only significant increase in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80903-5
更新日期:1984-10-15 00:00:00
abstract::The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80911-4
更新日期:1984-10-15 00:00:00
abstract::Recently, reports have appeared which show structural variations in B-DNA and indicate deviations from a uniform helical structure. We report for the first time that these indications are also present in the B-form fibre diffraction patterns for the lithium salt of natural DNA. We have used an improved method of contr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80922-9
更新日期:1984-10-15 00:00:00
abstract::'Peripheral' binding sites for benzodiazepines are under neural or homonal control in the pineal gland, olfactory bulb, and kidney. These observations prompted a search for an endogenous substance which could modulate these sites under physiological conditions. Acidified methanol extracts from several tissues (e.g. st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80913-8
更新日期:1984-10-15 00:00:00
abstract::Electron microscopy of mammalian smooth muscle myosin rods showed them to be 153 +/- 7 nm (SD) long, and to bend sharply (greater than 90 degrees) but infrequently, and pH independently (range 6.5-9.5), at a single site 45 +/- 4 nm from one end of the molecule. Light meromyosin (LMM) preparations were 99 +/- 10 nm lon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80940-0
更新日期:1984-10-15 00:00:00
abstract::The possible molecular heterogeneity of human transferrin receptors was analyzed using two murine monoclonal antibodies, Tü15 and Tü67. Both reagents precipitated from lysates of 125I-labeled HL-60 cells a major component of 88 kDa which could be identified as the transferrin receptor by comparison with the proteins d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80751-6
更新日期:1984-10-01 00:00:00
abstract::It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80767-x
更新日期:1984-10-01 00:00:00
abstract::Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80778-4
更新日期:1984-10-01 00:00:00
abstract::Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80784-x
更新日期:1984-10-01 00:00:00
abstract::The complete sequence of a 94 amino acid human seminal plasma polypeptide exhibiting inhibin-like activity is presented. This molecule, called beta-inhibin, selectively and specifically suppresses the release of pituitary FSH in vivo as well as in vitro. It does not affect the secretion of LH. Such a novel acidic prot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80766-8
更新日期:1984-10-01 00:00:00
abstract::Growth of regenerating lung lobes is preceded by an increase of the activity of L-ornithine decarboxylase (ODC). Mechanical factors play a role in determining both the growth rate and enhancement of activity of ODC in regenerating lung lobes. Hormonal regulation also appears of importance as shown by the increased ODC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80585-2
更新日期:1984-09-17 00:00:00
abstract::The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80558-x
更新日期:1984-09-17 00:00:00
abstract::Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80572-4
更新日期:1984-09-17 00:00:00
abstract::A peroxidase with stability, chromatographic and immunoreactive properties similar to that of bovine lactoperoxidase has been partly purified from human colostrum. Hydrophobic interaction chromatography on Phenyl-Sepharose C1-4B gave a 10-fold purification with an apparent recovery of about 45%. The enzyme was quantit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81177-1
更新日期:1984-09-03 00:00:00
abstract::The effects of the quaternary agent meproadifen on ACh-activated channel currents were studied on myoballs cultured from hind limb muscles of neonatal rats. Meproadifen (0.02-0.1 microM) combined with ACh (0.1-0.3 microM) in the patch pipette caused an increase, followed by a decrease, in the frequency of channel open...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81171-0
更新日期:1984-09-03 00:00:00
abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81172-2
更新日期:1984-09-03 00:00:00
abstract::We have investigated the action of hydrocortisosone on arachidonic acid mobilisation in cultures of mouse peritoneal macrophages, mouse L929 cells and the mouse macrophage-like cell line RAW264. Hydrocortisone inhibits both arachidonic acid release and prostaglandin production by L929 cells. However, prostaglandin pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81093-5
更新日期:1984-08-20 00:00:00
abstract::The binding of Mg2+ ADP to both rabbit skeletal and bovine cardiac myofibrils has been studied at two different temperatures. In each case a single class of binding sites was observed with a binding constant very close to that reported for the analogous actomyosin-subfragment one but much weaker than that seen with th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81067-4
更新日期:1984-08-20 00:00:00
abstract::Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81083-2
更新日期:1984-08-20 00:00:00
abstract::The phorbol esters are among the most potent tumor promoters. On addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) to isolated human platelets prelabelled with [32P]orthophosphate we found a rapid increase in 32P incorporation into phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. In vie...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80811-x
更新日期:1984-08-06 00:00:00
abstract::Published amino acid sequences for nitrogenase component polypeptides were compared with those of other proteins which also bind adenine nucleotides. Three sequences which might contribute to an adenine nucleotide-binding domain were found for the Fe-protein component of nitrogenase. The beta-subunit of the MoFe-prote...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80812-1
更新日期:1984-08-06 00:00:00
abstract::Side chain-hydroxylated derivatives of cholesterol (OH sterol) inhibiting lymphoblastic transformation bind with high affinity and specificity to a hydroxysterol binding protein (OHSBP) in the cytosol of human lymphocytes. These binding properties of OHSBP suggested some analogies with that of steroid hormone receptor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80798-x
更新日期:1984-08-06 00:00:00
abstract::Islet-activating protein (IAP) suppressed Ca2+-induced histamine release and phospholipase A2 activation in Gpp(NH)p-loaded mast cells. Since the guanine nucleotide-binding protein involved in adenylate cyclase inhibition is known to lose its function upon being ADP-ribosylated by IAP, the nucleotide-binding protein i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80816-9
更新日期:1984-08-06 00:00:00
abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81015-7
更新日期:1984-07-23 00:00:00
abstract::Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81037-6
更新日期:1984-07-23 00:00:00
abstract::Treatment of rat thymus cell suspensions with dexamethasone resulted in inhibition of engaged RNA polymerase A, without significant change in free pool activity. Studies with the re-initiation inhibitor, rifamycin AF/0-13, and measurements of numbers of RNA polymerase A molecules and of elongation rates showed that th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81031-5
更新日期:1984-07-23 00:00:00
abstract::The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81052-2
更新日期:1984-07-23 00:00:00
abstract::Human red cells suspended in lead-citrate buffers (2.6 microM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0 degrees C. Tracer studies with 203Pb indicate that the low uptake at 37 degrees C in the presenc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81135-7
更新日期:1984-07-09 00:00:00
abstract::Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81148-5
更新日期:1984-07-09 00:00:00
abstract::Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80884-4
更新日期:1984-06-25 00:00:00